黄新新;朱静;李璇玥;马田;李晓斌;李佳豪;丁峰;高慧香;

• 1:新疆农业大学动物科学学院
• 2:新疆农业大学计算机与信息工程学院

摘要(Abstract)：

试验旨在研究石榴皮瓤提取物中的鞣花酸对小鼠盲肠菌群多样性的影响,为深层次研究鞣花酸调控肠道菌群的生物学作用提供理论依据。试验选择平均日龄50 d、体重相近(37.31±5.57)g、健康良好的SPF级雄性昆明小鼠30只,随机分为2组,每组15只,分别为对照组和试验组。在相同基础日粮条件下试验组小鼠灌喂1.5 g/(d·只)石榴皮瓤粗提物,对照组小鼠灌喂相应体积的蒸馏水,进行为期21 d的灌喂试验。试验结束当天,屠宰全部试验小鼠,采集盲肠内容物并检测相关指标。试验结果显示:①在门水平菌群变化中,试验组小鼠肠道中拟杆菌门丰度显著高于对照组,比对照组提高了65.00%(P<0.05),且降低了厚壁菌门/拟杆菌门的比值;显著降低了放线菌门的丰度(P<0.05)。②在科水平菌群变化中,显著增加了理研菌科的丰度(P<0.05);减少了毛螺菌科的丰度,试验组比对照组降低了41.65%(P>0.05)。③在属水平菌群变化中,显著增加了拟杆菌属,试验组比对照组增加了96.84%(P<0.05);且肠杆菌属试验组比对照组有所减少(P>0.05)。④试验组小鼠Shannon、chao1和ACE指数都高于对照组(P>0.05);试验组LEfSe分析有差异细菌物种10种,对照组3种。因此,给小鼠灌喂石榴皮瓤提取物能够显著改变小鼠肠道菌群的种类和丰度,并提高物种多样性。

关键词(KeyWords)： 鞣花酸;小鼠;盲肠;肠道菌群;多样性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金[31860649];; 天池博士计划项目[2018XJTC0012];; 大学生创新项目[2020]

作者(Author): 黄新新;朱静;李璇玥;马田;李晓斌;李佳豪;丁峰;高慧香;

Email:

DOI: 10.13302/j.cnki.fi.2021.03.005

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