郁彬琦;靳翠丽;刘青;周晓见;

• 1:扬州大学环境科学与工程学院
• 2:扬州大学海洋科学与技术研究所

摘要(Abstract)：

利用正交试验研究氮磷供给水平和盐度对杜氏盐藻物质生产能力的影响,发现磷源浓度的影响不显著,而培养基的盐度和氮源浓度影响显著。氮源充足有利于蛋白质生产;低盐度(20‰)有利于蛋白质和总糖生产;中等浓度的氮源(NaNO_3 18.75 mg/L)和高盐(60‰)有利于藻粉干重产出。作为饲料添加成分的高蛋白杜氏盐藻,可采取高盐、高氮培养。如考虑综合利用,对各条件合理组合,也可提高培养效益。

关键词(KeyWords)： 杜氏盐藻;正交试验;物质生产;培养条件;营养盐供给

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目[41776156、41271521];; 江苏省自然科学基金项目[BK20180940]

作者(Author): 郁彬琦;靳翠丽;刘青;周晓见;

Email:

DOI: 10.13302/j.cnki.fi.2021.03.010

参考文献(References)：

• [1]GHARIBI M R,NOORI A,AGH N,et al.Rainbow trout farm effluent as a potential source of feed and medium for mass culture of Artemia parthenogenetica[J].Aquaculture,2020,530:735714.
• [2]郑秀洁,刘亚培.盐藻的开发与应用展望[J].盐科学与化工,2015,44(1):1-4.
• [3]王海军,唐淑萍,马昆.工业化生产的盐湖生物--盐藻[J].中国盐业,2016(3):41-46.
• [4]HARUN R,SINGH M,FORDE G M,et al.Bioprocess engineering of microalgae to produce a variety of consumer products[J].Renewable&Sustainable Energy Reviews,2010,14(3):1037-1047.
• [5]SALAMA E S,HWANG J H,El-DALATONY M M,et al.Enhancement of microalgal growth and biocomponent-based transformations for improved biofuel recovery:A review[J].Bioresource Technology,2018,258:365-375.
• [6]郭金耀,杨晓玲.营养方式对盐藻生长与物质积累的影响[J].江苏农业科学,2007(1):138-140.
• [7]范丰梅,秦彩云,郑维发,等.紫外辐射对盐藻SZ-05的胞外多糖和β胡萝卜素产量的影响[J].江苏师范大学学报(自然科学版),2009,27(1):92-94.
• [8]SINGH S P,SINGH P.Effect of temperature and light on the growth of algae species:A review[J].Renewable and Sustainable Energy Reviews,2015,50:431-444.
• [9]姜玮,李赟,孙红梅.绿色杜氏藻的盐度耐受性及高盐胁迫对其蛋白表达的影响[J].中国海洋大学学报(自然科学版),2009(1):347-352.
• [10]王梓,惠伯棣,宫平.培养基初始p H值和照射光波长对杜氏藻中类胡萝卜素含量的影响[J].食品科学,2015(23):205-210.
• [11]LAMERS P P,JANSSEN M,DE VOS R C,et al.Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina,a unicellular green microalga[J].Journal of Biotechnology,2012,162:21-27.
• [12]PEREIRA S,OTERO A.Effect of light quality on carotenogenic and non-carotenogenic species of the genus Dunaliella under nitrogen deficiency[J].Algal Research-Biomass Biofuels and Bioproducts,2019,44:101725.
• [13]LIU Y,YILDIZ I.The effect of salinity concentration on algal biomass production and nutrient removal from municipal wastewater by Dunaliella salina[J].International Journal of Energy Research,2018,42(3):1-10.
• [14]FARHAT N,RABHI M,FALLEH H,et al.Optimization of salt concentrations for a higher carotenoid production in Dunaliella salina (chlorophyceae)[J].Journal of Phycology,2011,47(5):1072-1077.
• [15]LIU J.Optimisation of biomass and lipid production by adjusting the interspecific competition mode of Dunaliella salina and Nannochloropsis gaditana in mixed culture[J].Journal of Applied Phycology,2014,26:163-171.
• [16]李娟,张耀庭,曾伟,等.应用考马斯亮蓝法测定总蛋白含量[J],中国生物制品学杂志,2000,13(2):118-120.
• [17]罗毅,潘细贵,刘刚,等.苯酚-硫酸法测定多糖含量显色方式的优选[J],中国中医药信息杂志,2005,12(1):45-46.
• [18]高忠江,施树良,李钰.SPSS方差分析在生物统计的应用[J].现代生物医学进展,2008,8(11):2116-2120.
• [19]方珍珍,闫珊珊,白东清,等.盐藻、辣椒粉及温度对红白锦鲤生长及着色的影响[J].饲料工业,2014,35(18):24-29.
• [20]MA K,BAO Q,WU Y,et al.Evaluation of microalgae as immunostimulants and recombinant vaccines for diseases prevention and control in aquaculture[J].Frontiers in Bioengineering and Biotechnology,2020,8:590431.
• [21]杨育凯,陈效儒,林黑着,等.饲料盐藻水平对卵形鲳鲹体色和生长的影响[J].水产科学,2017,36(3):336-339.
• [22]郭金耀,杨晓玲.盐藻蛋白质的提取分离[J].食品科技,2010,35(8):232-235.
• [23]KADKHODAEI S,ABBASILIASI S,SHUN T J,et al.Enhancement of protein production by microalgae Dunaliella salina under mixotrophic conditions using response surface methodology[J].RSC Advances,2015,5(48):38141-38151.
• [24]孙协军,范靖晗,李秀霞,等.复合海藻多糖对干制中国对虾新鲜度及色泽的影响[J].食品工业,2018,39(2):91-94.
• [25]张云鹏,张慧,李慧,等.杜氏盐藻多糖的研究现状及应用前景[J].山东化工,2017(15):80-81.
• [26]孙协军,赵利爽,李秀霞.盐藻多糖对冻藏中国对虾水分分布和品质变化的影响[J].食品工业科技,2017,38(7):315-319.