刘又铭;房鑫;陈璐;宋倩倩;管庆丰;张海文;

• 1:海南大学动物科技学院
• 2:海南大学生命科学与药学院

摘要(Abstract)：

为了探讨绿色安全抗生素替代品牛乳源肽(Lactoferricin Bovine,Lfcin B)的抗菌活性、稳定性及其与抗生素协同效应。检测Lfcin B与三种抗生素对常见致病菌的最小抑菌浓度与最小杀菌浓度;采用棋盘法分析Lfcin B与抗生素联合使用效果;测定不同温度与pH值对Lfcin B抑菌活性影响。实验结果表明:Lfcin B和抗生素对常见畜禽致病菌均具有良好抑菌活性,对金黄色葡萄球菌的MIC值为8μg/mL;Lfcin B和阿莫西林联合使用存在协同效应,对大肠杆菌和猪霍乱沙门氏菌的分级抑制浓度指数(fractional inhibitory concentration index, FIC指数)达到0.312 5;Lfcin B的热稳定性和酸碱稳定性良好,在40～60℃和p H 6～8的条件下对金黄色葡萄球菌的MIC值保持在8μg/mL。结果提示,Lfcin B具有成为新型饲用抗生素替代品的巨大潜力。

关键词(KeyWords)： 牛乳源肽;抗生素;抑菌活性;协同杀菌效应;稳定性

Abstract:

Keywords:

基金项目(Foundation): 海南省自然科学基金资助[320RC463];; 海南省高等学校教育教学改革研究资助项目[Hnjg2020-23];; 海南大学教育教学改革研究项目[hdjy2026];; 海南省青年科技英才计划[QCXM2018];; 海南省重点研发项目[ZDYF2018085]

作者(Author): 刘又铭;房鑫;陈璐;宋倩倩;管庆丰;张海文;

Email:

DOI: 10.13302/j.cnki.fi.2021.03.009

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